A microplate lid and a heat seal were also tested for their UV absorbance. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Model:. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 11 10 100 1000 0 20 40 60 80 100 120100 µL (96-well) or 20 µL (384-well) distilled water, and its absorbance was measured from 200 nm to 400 nm in 1-nm increments. Alpha 384 STD 96- and 384-well . Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. x. 2. ± 0. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. 2 h of incubation at 37 °C, the absorbance of each well was determined using the microplate reader. No filter change with dual monochromator optics. absorbance, fluorescence, and luminescence with the added functionality of. For more sample throughput, the system canThe SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. 2% in 384 well : microplates: Optimized sensitivity (ATP Glow) 96 wells 2 pM:The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Molecular Devices. Molecular Devices SpectraMax® Plus 384 Microplate Reader and SoftMax® Pro Software to efficiently collect and analyze the data for enzymatic determination of residual sugar in wine. 384-Well Low Profile Microplate, round well, V-bottom, sterile, with cover. BRET and FRET detection 6- to 384-well microplate reader Optimized with Promega cell viability. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 003 fmol 0. ©2016 Molecular Devices, LLC 2/16 0120-1282E Printed in USA Compatible with these Molecular Devices systems Ordering information Item Description Part number CatchPoint cAMP 384-well Fluorescent Assay Kit (Explorer) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (2) 384-well. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. x. 384-WELL SBS STERILE MICROPLATE. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Absorbance microplate readers and spectrophotometers for visible and UV-visible absorbance detection used for many research applications including ELISAs, nucleic acid and protein quantitation, and microbial growth. DOI or PMID # N/A. We would like to show you a description here but the site won’t allow us. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. For more sample throughput, the system canBy using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Key applications: ELISA, nucleic acid, endotoxin testing. printed in u. 40266. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Documentation:Molecular Devices SpectraMax Plus 384 Microplate Reader, 190 – 1000 nm, Molecular Devices. Compare. #6590-0118) Filter 665/10 (Molecular Devices cat. 2 nm Wavelength Bandpass 2. 5577. Molecular Devices' microplate readers. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Compare SpectraMax® M5 Microplate Reader from Molecular Devices LLC on Biocompare. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. –Min. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Plate Shaking. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator. Validated by Molecular Devices for the FLIPR reader. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit 0200-7017. Spectranet customer portalSpectraMax® Microplate Reader User Manual Download Page. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. With various on-board features and. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Endpoint, kinetic, spectrum and area-well scanningThe Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Features. 더 많은. LVF Monochromator™ : Excitation and Emission. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. High sensitivity luminescence reader. validation, maintenance, and repair documentation. x. S. This ready-to-use multimode plate reader is. Fluid dispensing speed. Or scan up to 384 wells at any time to determine the best assay conditions. Detection modalities are shown in Table 1-1. FlexIII. An example of dual-luciferase assay results. This is equivalent to having 511 built-in filters,This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 5 mP 1536–2. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The trademarks used herein are the property of Molecular Devices, LLC or. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). For more sample throughput, the system canSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. [Carbachol] (nM) RFU Max. Microplates . Includes the world’s most published microplate reader control and data analysis. in a 96-well microplate. This is equivalent to having 511 built-in filters,Step 1: Turn on your microplate reader. #MSB1001) SpectraMax i3xMulti-Mode Microplate Reader (Molecular Devices cat. Detection. Learn more about Microplate Readers. Fast and compact absorbance microplate readers for a wide range of assays without the use of filters96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4 64-Well Micro-Volume Sample Slide SpectraDrop_QSG. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Also see: SoftMax® Pro Microplate Software User Manual Download Page. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. This is equivalent to having 511 built-in filters,For nearly 30 years, Molecular Devices has provided scientists with tools to expand the boundaries of their research. Published Dec 16, 2020 | Updated Dec 16, 2020. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). Supplier: Molecular Devices. 96 Deep Well Microplate for KingFisher™ Systems . 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. 50 racks/case for 384-well (16 channel pipettor head. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. No accessories are needed and SoftMax® Pro Software. Temperature Control: ambient from +4 to 45C. 003 fmol 0. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. CLS3821. 1 pM 1536-well: 0. #1402-9700) 384-well low volume solid black microplate (Corning cat. flexibility of monochromator-based systems. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read None. Fluorescence Microplate Reader Comparison Chart. 010QS); use with a frame for 8-well strips, E & K Scientific Products (catalog # 564101). Plates with corners cut at. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Standard SBS 96 and 384 well plates (128. 과 같은 Parameter를 검사하여 평가할 수 있습니다. SpectraTest® Validation Package. Supplier: Molecular Devices. –Min. com. Description: The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Calcium flux was measured with a FlexStation 3 Microplate. A preconfigured. This is equivalent to having 511 built-in filters,For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Dimensions 128 x 86 x 15 mm. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. x. For biological research. Cross-talk < 0. Read one sample or up to 384 in a. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. < 0. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. x. 0. Find regional support. $542. Related Products: Low Binding Plates. As a result, assay flexibility is consequence-free. This unlocks the transport slide. All lab equipment in stock already tested and ships in 1-3 days. x. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Products : General, Microplate Readers. 95. 87. . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Minimum volume and maximum throughput with 96-. 7 at 10% conversion of 10 µM ATP; Read a 384-well plate in three minutes or less * Note: Programming the washer with a final aspirate height of 4. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Molecular Devices' microplate readers. both cuvette-based and microplate reader applications on the same instrument. Microplate reader assays requiring infrared (IR) or red-shifted. 12 x 75 tubes and 96-well or 384-well microplates can be used to analyze samples. Detection modalities are shown in Table 1-1. We would like to show you a description here but the site won’t allow us. On the back, in the right hand top corner, there. When the user applies a set temperature of 37°C, the top is heated to 37. Operators can also increase sample throughput by integrating the system into a complete robotic mechanism that can be easily adjusted to suit. The SpectraMax ABS. In the M2, M5 and Plus. Important things to consider when purchasing a multimode microplate reader. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Note that this procedure is only for the SpectraMax L model. Gold Standard and Reference Standard Molecular Devices microplate readers are used for recertification so that your certificate values are traceable. We would like to show you a description here but the site won’t allow us. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 1% in white 96 and < 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Includes PC and software. x. For more sample throughput, the system can The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The VersaMax and SpectraMax 190 read 96-well microplates. 11 10 100 1000 0 20 40 60 80 100 120The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 0A-Sunnyvale, CA-Made in USAElisa Plate Readers and Microplate Readers at Biocompare. Open the Template Editor for the plate. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Condition: Used - Good. Select Start>Control panel>Device Manager. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Multimode Reader Comparison Chart. TypeThe VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 5 mm. It was taken from a working lab. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. 8 mm distance between well bottoms to plate bottom; Validated by Molecular Devices for the FLIPR reader. between the affordability of filter-based readers and the . #3676) Microseal B PCR plate sealing film (Bio-Rad cat. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. For more sample throughput, both readers can be easily integrated into full robotic 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. For more sample throughput, the system canMicroplate readers. Elisa Plate Readers and Microplate Readers at Biocompare. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. All stand-alone instrument functions can beTo address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. flexibility of monochromator-based systems. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. Optical System: High energy xenon flash lamp. The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. In . 11 10 100 1000 0 20 40 60 80 100 120Next generation SpectraMax® absorbance microplate readers. San Jose, California, June 5, 2018. It measures the optical density (OD) of samples in both 96- and 384-well. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 3% in white 96- and 384-well microplates Time. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. A. Microplate format. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Features: LMax II Measures Glow Luminescence; Microplate Formats: 96 and 384 well; Temperature Control: 5-45C; Sensitivity: < 20 amol ATP; Two Injectors for Both Flash and Glow Luminescence Adjustable in 10-μl steps between 10 and 100 μlSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. LMax II. For more sample throughput, the system canThis Molecular Devices Lmax ll 384 Luminometer is a microplate reader. CLIPR. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. ) on MedicalExpo, the medical equipment specialist for your professional purchases. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. , April 14, 2022 – Molecular Devices, LLC. Instruments. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Luciferase standard curve in 384-well format. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette Flexibility to read a variety of plate formats. Title: SpectraMax i3 Multi-Mode Platform | Molecular Devices Author: Molecular Devices, LLCThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Temperature control. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Back View All Sample Preparation. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC. Other Molecular Devices readers with absorbance detection mode, e. It is cost and time effective. Efficient cell washing with minimal cell loss and optimal assay performance. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. SpectraMax ABS Plus Microplate Reader (Molecular. The FlexStation 3 reader combines Molecular Devices. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Top or bottom detection is available with a simple click of a button. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 6/10 #0120. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Select Update Driver. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. g. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. It also integrates with the. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. 19 H mm that meet microplate industry standards. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. x. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 100 fmol 384-well (75 µL) 0. For more sample throughput, the system canSpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 020 fmol 0. Simplified, homogeneous experimental protocol. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 76 L x 85. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Made from polypropylene with conical well profiles to. Microplate Reader. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. [Carbachol] (nM) RFU Max. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. This reader's dual monochromators for. For more sample throughput, the system canPowered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per second and quickly. Plates with corners cut at A1 and H1 positions to conform to SBS nomenclature. Four wash/rinse bottles are included and configurable for both 96- and 384. 0200-6263. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. Over a period of 24 hours, changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Efficiency. Access all the resources, tools and information you need to stay productive. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. s. 1). It also allows for the elimination of standard curves. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. SpectraMax® 340PC384 Microplate Reader for. x. 1/17 1907B Printed in USA . SoftMax Pro. This is equivalent to having 511 built-in filters,SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. Method. Add 150 µL reagent to each well. SpectraMax i3x Multi-Mode Microplate Reader. 7°C (gradient heating design). Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. [Carbachol] (nM) RFU Max. Create custom fluorescence assays without having to purchase expensive filter sets. Shaker time 0 to 999 seconds. The CellTiter-Glo assay from Promega uses luciferase. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Brand: MOLECULAR DEVICES. benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 7°C and the bottom to 36. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, FlexStation 3. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. It. A single-mode plate reader is a good starting point. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range: SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) 384-well solid white plates, Greiner (Cat# 655075) CHO-K1 cells, ATCC (Cat# CCL-61) Complete Growth Media Ham’s F12 Medium, Life Technologies (Cat# 11765-54) the iD5 reader’s filter system. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. x. 0 mP 384–1. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. For more sample throughput, the system can 384-well plates • Automated rinse reduces clogging • Quiet and efficient. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. 384 wells < 45 sec.